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    ATCC strain parent strain relevant genotype main glycan modification
    Strain Parent Strain Relevant Genotype Main Glycan Modification, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    strain parent strain relevant genotype main glycan modification - by Bioz Stars, 2026-09
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    Glycoproteomics:

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
    Article Snippet: .. Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study. .. First, the pCRII-pgl plasmid was constructed by PCR amplifying the whole N400 pgl locus with primers pgl-F (5′-AGCATATTGACGGGCTTGTCGC-3′) and pgl-R-(5′-AAGCGAAATCCTCGGACACG-3′) and inserting the PCR product into the pCRII-TOPO vector (Invitrogen).

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
    Article Snippet: .. Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study. . Allelic exchange of pglB/pglB2 . ..

    Modification:

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
    Article Snippet: .. Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study. .. First, the pCRII-pgl plasmid was constructed by PCR amplifying the whole N400 pgl locus with primers pgl-F (5′-AGCATATTGACGGGCTTGTCGC-3′) and pgl-R-(5′-AAGCGAAATCCTCGGACACG-3′) and inserting the PCR product into the pCRII-TOPO vector (Invitrogen).

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
    Article Snippet: .. Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study. . Allelic exchange of pglB/pglB2 . ..



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    strain parent strain relevant genotype main glycan modification  (ATCC)
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    ATCC strain parent strain relevant genotype main glycan modification
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    Neisseria strains used in this study
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    Neisseria strains used in this study

    Journal: Journal of Bacteriology

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species ▿ †

    doi: 10.1128/JB.00101-10

    Figure Lengend Snippet: Neisseria strains used in this study

    Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study Allelic exchange of pglB/pglB2 .

    Techniques: Modification

    Neisserial glycoprotein profiles and glycan diversity. (Top panels) Immunoblotting of whole-cell lysates from strains of N. gonorrhoeae (Ngo) (N400 pglC, N400, and FA1090), N. meningitidis (Nme) (MC58, H44/76, Z2491, 8013, FAM18, BZ 10, and BZ 198), and N. lactamica (Nla) (ST-3787 and ST-640) with glycan-specific monoclonal antibodies. (Bottom panels) Immunoblotting using the SM1 MAb specifically recognizing class I pilin types and a polyclonal antiserum raised against the PilE-derived peptide 44KSAVTEYYLNHGKWPENNTSA64, which reacts with all pilin types. Thus, strains FAM18, BZ 10, ST-3787, and ST-640 all express class II pilins. Also, strains 8013, FAM18, BZ 10, and BZ 198 carry the pglB2 allele that is associated with synthesis of GATDH glycans, while all of the other strains have the pglB allele that is associated with synthesis of DATDH glycans.

    Journal: Journal of Bacteriology

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species ▿ †

    doi: 10.1128/JB.00101-10

    Figure Lengend Snippet: Neisserial glycoprotein profiles and glycan diversity. (Top panels) Immunoblotting of whole-cell lysates from strains of N. gonorrhoeae (Ngo) (N400 pglC, N400, and FA1090), N. meningitidis (Nme) (MC58, H44/76, Z2491, 8013, FAM18, BZ 10, and BZ 198), and N. lactamica (Nla) (ST-3787 and ST-640) with glycan-specific monoclonal antibodies. (Bottom panels) Immunoblotting using the SM1 MAb specifically recognizing class I pilin types and a polyclonal antiserum raised against the PilE-derived peptide 44KSAVTEYYLNHGKWPENNTSA64, which reacts with all pilin types. Thus, strains FAM18, BZ 10, ST-3787, and ST-640 all express class II pilins. Also, strains 8013, FAM18, BZ 10, and BZ 198 carry the pglB2 allele that is associated with synthesis of GATDH glycans, while all of the other strains have the pglB allele that is associated with synthesis of DATDH glycans.

    Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study Allelic exchange of pglB/pglB2 .

    Techniques: Western Blot, Derivative Assay

    Glycoprotein profiles and glycan antigenicity in strains N400, FA1090, and MC58. (A) Whole-cell lysates of N. gonorrhoeae N400 and FA1090 wild-type (wt) strains and pglA (monosaccharide-expressing) and pglC (glycosylation-null) mutants probed with MAb npg1. The strains used were KS100 (N400 wt), KS104 (N400 pglC), KS141 (N400 pglA), KS302 (FA1090 pglA), KS301 (FA1090 pglC), and KS300 (FA1090 wt). (B) Immunoblotting of whole-cell lysates of N. gonorrhoeae FA1090 and N. meningitidis MC58 wild-type strains and pglA (monosaccharide-expressing) and pglC (glycosylation-null) mutants probed with MAbs npg1, npg2, and npg3. The strains used were KS301 (FA1090 pglC), KS302 (FA1090 pglA), KS300 (FA1090 wt), KS317 (MC58 pglC), KS318 (MC58 pglA), and KS316 (MC58 wt).

    Journal: Journal of Bacteriology

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species ▿ †

    doi: 10.1128/JB.00101-10

    Figure Lengend Snippet: Glycoprotein profiles and glycan antigenicity in strains N400, FA1090, and MC58. (A) Whole-cell lysates of N. gonorrhoeae N400 and FA1090 wild-type (wt) strains and pglA (monosaccharide-expressing) and pglC (glycosylation-null) mutants probed with MAb npg1. The strains used were KS100 (N400 wt), KS104 (N400 pglC), KS141 (N400 pglA), KS302 (FA1090 pglA), KS301 (FA1090 pglC), and KS300 (FA1090 wt). (B) Immunoblotting of whole-cell lysates of N. gonorrhoeae FA1090 and N. meningitidis MC58 wild-type strains and pglA (monosaccharide-expressing) and pglC (glycosylation-null) mutants probed with MAbs npg1, npg2, and npg3. The strains used were KS301 (FA1090 pglC), KS302 (FA1090 pglA), KS300 (FA1090 wt), KS317 (MC58 pglC), KS318 (MC58 pglA), and KS316 (MC58 wt).

    Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study Allelic exchange of pglB/pglB2 .

    Techniques: Expressing, Western Blot

    Increased npg2 and npg3 immunoreactivity at high pH and in pglI mutants. Immunoblotting of whole-cell lysates of wild-type strains, pglI mutants, and high-pH-treated wild-type strains revealed increased reactivity with the npg2 and npg3 monoclonal antibodies due to the lack of an O-acetyl group in pglI mutants or with the high-pH treatment. The extraneous band that appeared during high-pH treatment is PilE (as determined by immunoblotting with SM1 [data not shown]). This band was not present with the 4/3/1 background, in which pilE was conditionally repressed. The strains used were KS100 (N400 wt), KS144 (N400 pglI), KS104 (N400 pglC), KS101 (4/3/1 wt), KS300 (FA1090 wt), KS303 (FA1090 pglI), and KS301 (FA1090 pglC). wt, wild type.

    Journal: Journal of Bacteriology

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species ▿ †

    doi: 10.1128/JB.00101-10

    Figure Lengend Snippet: Increased npg2 and npg3 immunoreactivity at high pH and in pglI mutants. Immunoblotting of whole-cell lysates of wild-type strains, pglI mutants, and high-pH-treated wild-type strains revealed increased reactivity with the npg2 and npg3 monoclonal antibodies due to the lack of an O-acetyl group in pglI mutants or with the high-pH treatment. The extraneous band that appeared during high-pH treatment is PilE (as determined by immunoblotting with SM1 [data not shown]). This band was not present with the 4/3/1 background, in which pilE was conditionally repressed. The strains used were KS100 (N400 wt), KS144 (N400 pglI), KS104 (N400 pglC), KS101 (4/3/1 wt), KS300 (FA1090 wt), KS303 (FA1090 pglI), and KS301 (FA1090 pglC). wt, wild type.

    Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study Allelic exchange of pglB/pglB2 .

    Techniques: Western Blot

    Protein glycosylation genotypes and phenotypes

    Journal: Journal of Bacteriology

    Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species ▿ †

    doi: 10.1128/JB.00101-10

    Figure Lengend Snippet: Protein glycosylation genotypes and phenotypes

    Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Parent strain Relevant genotype Main glycan modification (identified by MS) Reference N. gonorrhoeae strains FA1090 (ST-1899) ATCC 700825 KS300 (FA1090) FA1090 recA6 Ac-Hex-Hex-DATDH 59 KS301 FA1090 pglC :: kan No glycan This study KS302 FA1090 pglA :: kan DATDH This study KS303 FA1090 pglI :: kan Hex-Hex-DATDH This study KS100 (N400) VD300 recA6 Ac-Hex-DATDH 59 KS104 (GGC) N400 pglC :: kan No glycan 22 KS141 N400 pglA :: kan DATDH 2 KS142 N400 pglE on Ac-Hex-Hex-DATDH 2 KS144 N400 pglI :: kan Hex-DATDH 2 KS304 N400 pglE on pglI :: kan Hex-Hex-DATDH This study KS305 N400 pglB MC58 Ac-Hex-DATDH This study KS306 KS305 pglB MC58 pglA :: kan DATDH This study KS307 KS142 pglB MC58 pglE on Ac-Hex-Hex-DATDH This study KS308 N400 pglB2 8013 Hex-GATDH This study KS309 KS308 pglB2 8013 pglA :: kan GATDH This study KS310 KS142 pglB2 8013 pglE on Hex-Hex-GATDH This study KS101 (4/3/1) VD300 pilE ind 71 KS105 4/3/1 pglC :: kan 64 KS122 4/3/1 pglA :: kan 64 KS127 4/3/1 pglE on 65 KS311 4/3/1 pglB2 8013 This study KS312 4/3/1 pglB2 8013 pglA :: kan This study KS313 4/3/1 pglB2 8013 pglE on This study KS314 4/3/1 pglI :: kan This study KS315 4/3/1 pglE on pglI :: kan This study N. meningitidis strains KS316 (MC58, ST-74) 58 KS317 MC58 pglC :: kan This study KS318 MC58 pglA :: kan This study KS319 (H44/76, ST-32) 26 KS320 (Z2491, ST-4) 44 KS321 (8013, ST-177) 33 KS322 (FAM18, ST-11) 6 KS323 (BZ 10, ST-8) 33 KS324 (BZ 198, ST-41) 33 N. lactamica strains KS325 (ST-3787) 5 , ATCC 23970 KS326 (ST-640) 5 Open in a separate window Neisseria strains used in this study Allelic exchange of pglB/pglB2 .

    Techniques: